Merck
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I1884

Sigma-Aldrich

Insulin-transferrin-sodium selenite media supplement

γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture

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Synonym(s):
ITS Supplement
NACRES:
NA.75

biological source

human plasma

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P01308

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... INS(3630)

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11074547001I2521I2771
Sigma-Aldrich

Sigma-Aldrich

I1884

Insulin-transferrin-sodium selenite media supplement

Insulin-Transferrin-Sodium Selenite Supplement suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)

Roche

11074547001

Insulin-Transferrin-Sodium Selenite Supplement

Sigma-Aldrich

Sigma-Aldrich

I2521

ITS+1 Liquid Media Supplement (100×)

Sigma-Aldrich

Sigma-Aldrich

I2771

ITS+3 Liquid Media Supplement (100×)

biological source

human plasma

biological source

human serum (transferrin), synthetic (sodium selenite)

biological source

-

biological source

-

sterility

γ-irradiated

sterility

non-sterile; 0.2 μm filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

lyophilized powder

form

lyophilized

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Insulin-transferrin-sodium selenite media supplement supports in vitro growth and maturation of follicles from oocyte and is routinely used in in vitro fertilization methods.
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

Application

Insulin-transferrin-sodium selenite media supplement has been used as:
  • medium 199 (M199) media supplement for cardiac myocytes culture
  • Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture
  • Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells

Other Notes

Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Quantity

Each vial sufficient to prepare 5 liters of medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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