Merck
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L0288

Sigma-Aldrich

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
ACF lipid mixture
NACRES:
NA.75

biological source

non-animal source

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

2-8°C

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Solvent Mixture 20:9:1 chloroform:methanol:water

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Solvent Mixture 65:35:8 chloroform:methanol:water

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Solvent Mixture 65:35:8

biological source

non-animal source

biological source

-

biological source

-

biological source

-

form

liquid

form

solid

form

chloroform:methanol:water

form

chloroform:methanol:water

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

-10 to -25°C

storage temp.

2-30°C

storage temp.

2-30°C

Quality Level

500

Quality Level

100

Quality Level

-

Quality Level

-

sterility

sterile-filtered

sterility

-

sterility

-

sterility

-

General description

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Application

Lipid Mixture 1, Chemically Defined has been used:

  • as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
  • as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
  • to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Other Notes

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificate of Origin

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