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L3287

Sigma-Aldrich

Escort IV Transfection Reagent

Lipid reagent for transient and stable transfection of mammalian and insect cells.

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Synonym(s):
Gene delivery
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid (aqueous solution)

usage

 mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

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This Item
S1452XTGHP-ROXTG360-RO
form

liquid (aqueous solution)

form

aqueous solution

form

liquid (aqueous solution)

form

liquid (aqueous solution)

concentration

1 mg/mL

concentration

-

concentration

-

concentration

-

technique(s)

transfection: suitable

technique(s)

-

technique(s)

transfection: suitable

technique(s)

transfection: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

-

General description

Escort IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort IV, see the Transfection Reagent Selection Guide.

Features and Benefits

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of cell lines
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9, Sf21 and S2 insect cells

Components

Escort IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort IV is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
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Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
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beta-catenin is the central signalling molecule of the canonical Wnt pathway, where it activates target genes in a complex with LEF/TCF transcription factors in the nucleus. The regulation of beta-catenin activity is thought to occur mainly on the level of
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Articles

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

The product bulletin providin detailed use protocol for easy DNA transfection.

Product manual provides detailed protocol for easy DNA transfection.

Our Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. This is a fast and easy protocol is compatible with serum, serum-free medium and antibiotics.

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Related Content

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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