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L5520

Sigma-Aldrich

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Leibovitz L15
MDL number:
MFCD00217482
NACRES:
NA.75

Quality Level

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

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This Item
L1518L438651322C
Sigma-Aldrich

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L5520

L-15 Medium (Leibovitz)

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L1518

L-15 Medium (Leibovitz)

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L4386

L-15 Medium (Leibovitz)

SAFC

SAFC

51322C

Medium 199 Modified

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

components

phenol red: 0.011 g/L
L-glutamine: 0.3 g/L
sodium pyruvate: 0.55 g/L

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

components

L-glutamine: 100 mg/L
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
NaHCO3: 350 mg/L
phenol red: 21.24 mg/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Reconstitution

Supplement with 0.3 g/L L-glutamine.

also commonly purchased with this product

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supplement

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Description
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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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T1503
Product Number
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25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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