L9655

Linoleic Acid-Oleic Acid-Albumin, 100x

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): Fatty Acid-Albumin-complexes
• NACRES: NA.75

Properties

• biological source: protein from bovine
• Quality Level: 400
• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• composition: linoleic acid and oleic acid, 2 mol/mol albumin (each)
• concentration: 100 mg/mL BSA in DPBS
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C

Description

General description

Fatty Acid-Albumin-complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) is beneficial to the growth of a variety of cell types (e.g., baby hamster kidney (BHK) hybridoma). A mixture of poly-unsaturated and monosaturated fatty acids (i.e., linoleic acid and oleic acid) produces synergistic effect when used as a media supplement.

Application

Linoleic Acid-Oleic Acid-Albumin, 100x has been used:

• to induce acute hepatocellular stress in primary hepatocytes
• to induce oxidative stress in cytotrophoblasts
• to induce lipid droplet accumulation in HepG2 cells

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves