M9956

M9 Minimal Salts, 5X

Liquid microbial growth medium

• Synonym(s): 5x M9 salts
• NACRES: NA.85
• UNSPSC Code: 12352302
• Grade: Molecular Biology
• Form: liquid

Properties

• grade: Molecular Biology
• sterility: sterile; 0.2 μm filtered
• form: liquid
• suitability: suitable for nonselective for Escherichia coli and Coliforms
• storage temp.: room temp
• Quality Level: 500

Description

General description

M9 minimal media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.

Application

M9 Minimal Salts, 5X is suitable for non-selective cultivation of Escherichia coli strains for cloning, DNA plasmid production and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.[1][2]

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

M9 minimal media provides:

• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

Other Notes

15 g/L KH₂PO₄
64 g/L Na₂HPO₄ ⋅7H₂O
2.5 g/L NaCl
5.0 g/L NH₄Cl

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable