Merck
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N3520

Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
Ham’s F-12
MDL number:
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
N6760F6636N6658
SAFC

SAFC

F6636

Nutrient Mixture F-12 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

components

HEPES: no
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: yes
NaHCO3: no

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

differentiated cell culture medium, primary human liver cell cultuer medium

Low serum media

hybrid cell cultue, amino acid enriched

Ham′s F12, hepatocyte cultures, epithelial cell cultures, clonal toxicity assays, CHO cells

General description

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Application

Nutrient Mixture F-12 Ham is used for the following applications:
  • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
  • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
  • Used in media for the propagation of Cowdria ruminantium
  • Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Quantity

Formulated to contain 11.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.5 g/L sodium bicarbonate.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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