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N6013

Sigma-Aldrich

Nutrient Mixture F-10 Ham

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Ham’s F-10
MDL number:
MFCD00217607
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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N6013

Nutrient Mixture F-10 Ham

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Nutrient Mixture F-12 Ham

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N6658

Nutrient Mixture F-12 Ham

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N6635

Nutrient Mixture F-10 Ham

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

F-10 Ham supports the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues. Ham′s Nutrient Mixtures are developed to support the clonal growth of several clones like Chinese hamster ovary (CHO) cells, clones of HeLa, and mouse L-cells.

Application

Nutrient Mixture F-10 Ham has been used:

  • to culture lamina cribrosa explants
  • in in vitro culture of lizard tail regenerate
  • for culturing of lizard embryo-fibroblasts

Reconstitution

Supplement with 0.146 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Elisabetta Polazzi et al.
Tissue & cell, 43(6), 350-358 (2011-08-30)
An in vitro system of isolated skin cells has been developed in order to address the understanding on the factors that control the shedding cycle and differentiation of lizard epidermis. The skin from the regenerating lizard tail has been separated
Navita N Lopez et al.
Experimental eye research, 197, 108103-108103 (2020-06-12)
The lamina cribrosa is the initial site of glaucomatous injury. Pathological changes to the lamina cribrosa include posterior displacement of the lamina cribrosa, loss of trophic support, and remodeling of the extracellular matrix. Optic nerve head (ONH) astrocytes and lamina
Roser Buscà et al.
BMC evolutionary biology, 15, 179-179 (2015-09-04)
The Ras/Raf/MEK/ERK signaling pathway is involved in essential cell processes and it is abnormally activated in ~30 % of cancers and cognitive disorders. Two ERK isoforms have been described, ERK1 and ERK2; ERK2 being regarded by many as essential due

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