Merck
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N6658

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Ham’s F-12
MDL number:
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
N6013N4888N6635
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

HEPES: no
phenol red: yes
L-glutamine: no
sodium pyruvate: 0.11 g/L
NaHCO3: yes

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

components

HEPES: no
NaHCO3: no
sodium pyruvate: 0.11 g/L
L-glutamine: yes
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
  • to culture LAN-1 cells
  • to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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