Merck
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O1391

Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

Synonym(s):
Sudan Red 5B, Solvent Red 27
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
EC Number:
NACRES:
NA.47

form

liquid

Quality Level

concentration

0.5% in isopropanol

εmax

0.20-0.50 at 510-517 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

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General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Packaging

500 mL in glass bottle

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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