for molecular biology
7.7-8.1 (25 °C)
human ... GABRA1(2554)
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Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
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The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
The top phase is Tris buffer, so use the lower/bottom phase.
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
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