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P5188

Sigma-Aldrich

TWEEN® 80

for molecular biology, syrup

Synonym(s):
POE (20) sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polysorbate 80
CAS Number:
MDL number:
NACRES:
NA.21

Quality Level

grade

for molecular biology

description

non-ionic

form

syrup

mol wt

average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

CMC

0.012 mM

transition temp

cloud point 65  °C

density

1.06 g/mL at 25 °C

HLB

15

suitability

molecular biology tested

foreign activity

DNAse (Exonuclease Detection), NICKase (Endonuclease Detection), RNAse, none detected

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Packaging

100 mL in poly bottle

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ice Cream, 86-86 (2003)
Wei-Ju Chung et al.
Journal of biological physics, 43(4), 511-524 (2017-09-10)
DNA samples are commonly frozen for storage. However, freezing can compromise the integrity of DNA molecules. Considering the wide applications of DNA molecules in nanotechnology, changes to DNA integrity at the molecular level may cause undesirable outcomes. However, the effects
Fats in Food Technology (2014)
Qun Wang et al.
Brain research, 1285, 188-195 (2009-06-17)
The group IIA secretory phospholipase A2 (sPLA(2)-IIA) has been studied extensively because of its involvement in inflammatory processes. Up-regulation of this enzyme has been shown in a number of neurodegenerative diseases including cerebral ischemia and Alzheimer's disease. PX-18 is a
Cristina Marcos-Arias et al.
BMC complementary and alternative medicine, 11, 119-119 (2011-11-29)
Candida-associated denture stomatitis is a frequent infectious disease. Treatment of this oral condition is difficult because failures and recurrences are common. The aim of this study was to test the in vitro antifungal activity of pure constituents of essentials oils.

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