Merck
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R1755

Sigma-Aldrich

Rhodamine B isothiocyanate

mixed isomers, BioReagent, suitable for protein labeling

Synonym(s):
RBITC
Empirical Formula (Hill Notation):
C29H30ClN3O3S
CAS Number:
Molecular Weight:
536.08
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

description

labeling efficiency with bovine albumin > 70%

Quality Level

product line

BioReagent

fluorescence

λex 543 nm; λem 580 nm in methanol

suitability

suitable for protein labeling

storage temp.

−20°C

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

InChI key

YVSWPCCVTYEEHG-UHFFFAOYSA-N

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General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:
  • Rhodamine isothiocyanate labeling
  • Rhodamine-labeled synthetic APF
  • Preparations of protein and fluorescent conjugates
  • Fluorescent red dye

Packaging

100, 500 mg in glass insert
1 g in glass bottle

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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