R4408

Chloramphenicol

Ready Made Solution, 100 mg/mL in ethanol: isopropanol (95:5), ≥98% (HPLC)

• Synonym(s): D-(-)-threo-2,2-Dichloro-N-[β-hydroxy-α−(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, D-(-)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]acetamide
• NACRES: NA.85

Properties

• Quality Level: 200
• assay: ≥98% (HPLC)
• form: solution
• concentration: 100 mg/mL in ethanol: isopropanol (95:5)
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• Mode of action: protein synthesis | interferes
• shipped in: dry ice
• storage temp.: −20°C

Description

Packaging

10 mL

10ml

Application

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

Biochem/physiol Actions

Mode of Action: Chloramphenicol inhibits bacterial protein synthesis by blocking the peptidyl transferase step by binding to the 50S ribosomal subunit and preventing attachment of aminoacyl tRNA to the ribosome. It also inhibits mitochondrial and chloroplast protein synthesis and ribosomal formation of (p)ppGpp, de-pressing rRNA transcription.

Mode of Resistance: Use of chloramphenicol acetyltransferase will acetylate the product and inactivate it.

Antimicrobial Spectrum: This is a broad spectrum antibiotic against gram-positive and gram-negative bacteria, and is used mainly for ophthalmic and veterinary purposes.

Preparation Note

This product is a ready made solution of 100 mg/mL in ethanol:isopropanol (95:5). It is shipped in dry ice and stored at -20°C.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Storage class (TRGS 510): Flammable liquids")

Safety Information

• Pictograms: GHS02,GHS05,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H318 - H351 - H361fd
• Precautionary Statements: P202 - P210 - P233 - P280 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 57.2 °F - closed cup
• Flash Point(C): 14.0 °C - closed cup