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T1503

Sigma-Aldrich

Trizma® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

description

aminopeptidase substrate

Assay

≥99.9% (titration)

form

crystalline

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)

absorption

≤0.05 at 290 nm at 40%

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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1 of 4

This Item
T466193350T6791
Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline

Sigma-Aldrich

T1503

Trizma® base

-
Trizma® base ≥99.9% (titration), crystalline

Sigma-Aldrich

T4661

Trizma® base

-
Trizma® base puriss. p.a., ≥99.7% (T)

Sigma-Aldrich

93350

Trizma® base

-
Trizma® base BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Sigma-Aldrich

T6791

Trizma® base

Premium Grade
form

crystalline

form

crystalline

form

crystalline

form

crystalline

pH

10.5-12

pH

10.0-11.5

pH

10.5-11.5 (25 °C, 10% in H2O)

pH

10.5-12.0 (1 M in H2O)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.)

mp

167-172 °C (lit.), 168-172 °C

mp

167-172 °C (lit.)

solubility

methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)

solubility

water: soluble (400 g plus 600 mL of water)

solubility

water: soluble (678 g/l at 20 °C)

solubility

H2O: 1 M, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

General description

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.

Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.

Application

Trizma® base has been used:
  • as a component of H buffer (cell dissociation buffer)
  • for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
  • as an assay buffer for reconstitution of extracted and dried protein samples
  • to prepare Tris-HCl buffer that is used to stabilize proteins
  • as a buffer to extract carotenoid from tubers
  • as a component of sample buffer during protein extraction prior to western blotting
  • as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
  • as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Features and Benefits

  • Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Can be used in Cell Biology, and Biochemical research

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

suggested gloves for splash protection

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Sigurdsson E.M., Calero M., and Gasset M, (eds.)
Amyloid Proteins: Methods and Protocols, 473-491 (2012)
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Tribological performance of polydopamine + Ag nanoparticles/PTFE thin films
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Protocols

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

To measure carboxypeptidase A activity, hippuryl-L-phenylalanine is used in a continuous spectrophotometric rate determination assay at 254 nm. Carboxypeptidase A hydrolyzes peptide bonds at C-terminal residues.

To measure luciferase activity, an assay that measures light emission with a luminometer is used. Firefly luciferase is used for detection and quantitation of ATP and as a reporter for genetic function.

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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