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U0631

Sigma-Aldrich

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

product line

BioXtra

Quality Level

Assay

98.5-101.5%

form

powder, crystals or pellets

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

7.5-9.5 (20 °C, 5 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 5 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.06 at 260 nm at 5 M
≤0.06 at 280 nm at 5 M

application(s)

diagnostic assay manufacturing

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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1 of 4

This Item
U6504U5378RDD034
Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Sigma-Aldrich

U0631

Urea

Premium Grade
Urea suitable for electrophoresis

Sigma-Aldrich

U6504

Urea

-
Urea powder, BioReagent, for molecular biology, suitable for cell culture

Sigma-Aldrich

U5378

Urea

Essential+ Grade
Urea free-flowing, Redi-Dri™, Bioreagent, ≥98%

Sigma-Aldrich

RDD034

Urea

-
form

powder, crystals or pellets

form

powder or crystals

form

powder

form

-

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

impurities

≤0.005% Ammonia

impurities

-

impurities

-

pH

7.5-9.5 (20 °C, 5 M in H2O)

pH

-

pH

-

pH

-

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

H2O: 100 mg/mL

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

solubility

-

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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