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Bright-Line Hemacytometer

supplied with two cover slips








General description

The Bright-Line Hemacytometer is molded from a single piece of thermal and shock-resistant glass. An H-shaped moat forms two counting areas, or plateaus. A "V" slash at the loading side of each plateau facilitates charing and reduces the possibility of overflow into the moat. Each plateau features enhanced Neubauer rulings.

The ruled surface is 0.1mm below the cover glass, limiting the volume of blood or fluid over a square mm at and over each of 400 squares (within the central square mm) to 0.00025 cu. mm. Contact of the flat, polished cover glass surfaces with cover glass supports produces an exact volume of fluid over the counting area. The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.

Replacement cover slips sold separately: Z375357


Bright-Line® Hemacytometer is used for cell counting. It may be used for determining the spore concentrations in spore suspension. It might also be used to count the number of oocytes present in stock solutions.

Legal Information

Brady is a registered trademark of Brady Worldwide, Inc.
Bright-Line is a trademark of Cambridge Instruments, Inc.

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the ruling pattern used for Product Z359629, Bright-Line Hemacytometer?

    The details for Product Z359629, Bright-Line Hemacytometer are below: Cell Depth: 0.100mm +/- 2% (1/10mm)Volume: 0.1 MicroliterRuling Pattern: Improved Neubauer, 1/400 Square mmRulings cover 9 square millimeters. Boundary lines of the Neubauer ruling are the center lines of the groups of three. (These are indicated in the illustration below.) The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is .00025 cubic mm. 

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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