Millipore® Sterile Filters

Steriflip® filtration devices with available funnel attachment

Sterile filtration devices are used to filter-sterilize aqueous solutions and prepare culture media. We provide small and large volume sterilizing filters for cell culture applications. With over 60 years of expertise in sterile filtration, Millipore® devices set the industry standard for high-performance membrane technology in sterile filtration.

Steriflip® filters

Compatible with 50 mL centrifuge tubes, Steriflip® filters eliminate the liquid transfer step by integrating directly into the sample tube. To filter, attach the unit to the 50 mL centrifuge tube containing your sample, flip, and apply vacuum. The filtrate collects directly into the attached 50 mL tube. Three membranes are available:

  • Millipore® Express® polyethersulfone (PES) membranes are ideal for low protein binding and exhibit faster flow rates with viscous solutions
  • Durapore® PVDF membranes offer the lowest protein binding available and are ideal for scale-up applications.
  • Nylon mesh membranes are best for large particle removal.
Stericap™ bottle top filters for sterile filtration

Stericap™ bottle-top filters

The Stericap™ Universal Bottle-Top Filter Device enables fast vacuum filtration into compatible bottles. Even viscous solutions filter efficiently with no clogging, so these filters are well-suited for serum-supplemented media. Stericap™ filter devices are compatible with vacuum-rated bottles or other receptacles with an inner neck diameter of 20 to 67 mm, providing maximum versatility, capacity, and filtration rate.

  • Millipore Express® PLUS PES membrane for fastest flow rates and low protein binding
  • Fits any vacuum-rated bottle from 20 to 67 mm in diameter for maximum versatility
  • High volume capacity reduces preparation steps
  • Easy-flow control button means the filter can be easily transferred between bottles without stopping the vacuum
  • Free of pyrogenic and cytotoxic contaminants for healthy cell culture and critical downstream applications
Sterivex® GP pressure filter unit for sterile filtration

Sterivex™ filters

Filter-sterilize volumes up to 2,000 mL using Sterivex™ pressure-driven filter units. With three membrane options and a variety of outlet connections, Sterivex™ filters can fit nearly any workflow and dispense into nearly virtually any storage container. Sterivex™ filters work with peristaltic pumps, syringes, or pressure vessels to eliminate the risk of contamination from decanting or pipetting.

  • Works with diverse pressure devices including syringes, peristaltic pumps and pressure vessels
  • Available with multiple membrane types to accommodate varying application needs with capacities up to 2 L
    • 0.22 µm Millipore Express PES membrane for filter sterilization with fastest flow rates
    • 0.22 µm Durapore® PVDF membranes for sterilization of aqueous solutions with very low protein binding
    • 0.45 µm Durapore® PVDF membranes for clarification of aqueous solutions
Steripak™ pump-driven filters for sterile filtration

Steripak™ filter units

Steripak™ filter units are designed for larger-scale pressure-driven filtration of tissue culture media with or without serum supplement. These filters are designed for single use and are not autoclavable. Steripak™ units are available in two maximum volumes to filter up to 10 L or 20 L of fluid. Both sizes are equipped with the low protein-binding, fast flow 0.22 µm pore Millipore Express® membranes. Units are supplied sterile and ready to connect to a pump or pressure vessel.

  • Millipore Express® PLUS PES membrane for fastest flow with low protein binding
  • Larger scale, pressure-driven filtration device for faster flow rates reduces the time needed for large-volume filtrations
  • 10 L and 20 L capacity sizes are optimized for maximum laboratory-scale filtration options
  • Manually operated top air vent to prevent air lock stop of flow
  • Filling bell for cleaner filling environment around the opening of the receptacle bottle

STERICUP® AND STERITOP® STERILE FILTER DEVICES

When reliable sterile filtration of media and buffers is critical, laboratories around the world rely on Stericup® devices. A full range of filter membrane materials, pore sizes, and filtration volumes have made Stericup® filters the leader in filtration for cell culture and other applications where sterility is critical. The next-generation Stericup® Quick Release vacuum filtration devices feature ergonomic design innovations that enhance usability and streamline filtration, while safeguarding vital cell cultures with the dependable performance of Millipore® membranes.

  • Industry-leading membranes with an application-optimized selection of materials and pore sizes
  • Low protein-binding and low fluid retention minimize loss of critical proteins and reagents
  • Stem cell-tested Millipore Express® PLUS PES filters safeguard growth media

Our commitment to sustainability inspired us to design a filter device that helps labs to go green, without compromising sterility. Stericup® E and Steritop® E filters attach directly to commercial media bottles, eliminating the plastic filter funnel to reduce plastic waste up to 48%, with a corresponding significant reduction in packaging, transportation, and storage impacts.



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