Protein Electrophoresis Gels & Buffers

Polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE are common techniques used for protein separation. Protein gels can be hand-casted or purchased as pre-cast gels for convenience. The percentage of acrylamide in the gel affects resolution of protein bands, with higher percentages of acrylamide useful for resolving low molecular weight proteins and lower percentages of acrylamide useful in resolving higher molecular weight protein bands. Gradient pre-cast gels cover a wider range of molecular weight sizes. Hand-casting gel reagents, pre-cast gels, and running buffer powders are available for your lab needs.



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mPAGE® Bis-Tris Precast Gels

mPAGE® Bis-Tris precast gels provide excellent protein resolution at a budget-friendly price. Offering high resolution of protein bands, mPAGE® gels require shorter run times and are compatible with large sample volumes, making mPAGE® precast gels a valuable tool for protein research.

Features:

  • Publication-ready resolution at a fraction of the cost
  • Compatible with popular electrophoresis tanks
  • Up to 80 μL sample per well
  • Efficient wet and semi-dry Western blot transfer
  • Up to 15-minute shorter run time
  • Neutral pH prevents protein modification
  • Durable to prevent tearing during post-electrophoresis processing
  • Excellent compatibility with the popular gel running equipment and tanks including Thermo/Invitrogen, Bio-Rad®, and others

Turbomix™ Bis-Tris Gel Casting Solutions

Cast your own polyacrylamide gels using TurboMix™ Bis-Tris kits and solutions. TurboMix™ Bis-Tris Gel Casting Kits eliminate the variability and time-consuming nature of hand casting gels by supplying pre-mixed buffer and acrylamide solutions.

Features:

  • Premixed and ready-to-pour acrylamide buffer solutions reduce hands-on prep time
  • Quick Cast protocol allows simultaneous polymerization of resolving and stacking gels
  • Neutral pH running conditions result in reduced protein modification and sharper bands compared to standard Tris-glycine gels
  • Easily dilute to any required percentage acrylamide gel between 8% and 15%, to separate proteins 6 kDa – 400 kDa in size
  • Electrophoresis in as little as 20 minutes
  • Longer cast gel shelf life (3-4 weeks, with proper storage)

Running Buffers for Bis-Tris Gels

Two buffers that can be used as running buffers for SDS-PAGE gels: MES and MOPS. MES has a lower pKa than MOPS, which enables faster run times. MES buffer gives better separation of proteins at lower molecular weights, while MOPS buffer provides better separation at higher molecular weights. MES and MOPS SDS buffer powders are available for fast and easy preparation of running buffer for protein gel electrophoresis.

Other Reagents for Pouring Gels by Hand

PAGE and SDS-PAGE gels can be hand-casted using acrylamide/bisacrylamide with TEMED and ammonium persulfate to polymerize the gel. The reagents are prepared, mixed together, then poured between two glass plates to polymerize. Acrylamide and bisacrylamide are neurotoxins in solution, so care should be taken to avoid direct contact. High purity TEMED, ammonium persulfate, and bisacrylamide and acrylamide in powder or solution form are available for hand casting gels.

TruPAGE™ Precast Gels

TruPAGE™ SDS-PAGE precast gels provide high protein resolution and an extended shelf-life. TruPAGE™ gels are formulated with triethanolamine (TEA)-tricine, featuring a neutral pH running environment that helps prevents protein modification during the experiment.

Features:

  • Long shelf life (2 years from the date of manufacture when stored properly)
  • Extended full-length resolving gels provide improved resolution
  • Extra tall wells to prevent sample spill over and accommodate larger sample loading volumes
  • Excellent compatibility with the popular gel running equipment, including tanks from other manufacturers such as Thermo/Invitrogen, Bio-Rad®, and others
  • Fast run times as low as 30 minutes.