Detection Substrates & Enzymes
Detection substrates and enzymes are crucial in many areas of life science, both as tools and targets in detection systems. Our enzyme substrates and substrate detection systems offer convenience, stability, and consistency. They are routinely analyzed in our laboratories for identity, assay, and homogeneity, as well as for functional performance in the corresponding enzyme system. We offer substrates, buffers, and reagents in powder, liquid, tablet, and capsule forms for use in enzyme assays, ELISA, blotting, imaging, and many other applications. Our high purity enzymes have undergone extensive characterization and offer lot-to-lot consistency. Explore our enzyme products and discover ways to utilize our detection substrates and enzymes in different detection methods.
Products
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Cell Counting Kit - 8
for quantitation of viable cell number in proliferation and cytotoxicity assays
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
≥98% (HPLC), chromogenic, powder
Peroxidase from horseradish
Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Adenosine 5′-triphosphate disodium salt hydrate
BioXtra, ≥99% (HPLC), from microbial
Peroxidase from horseradish
Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol)
3, 3′,5 ,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, aqueous solution
OPD
SIGMAFAST™, peroxidase substrate, chromogenic, tablet
ABTS™ Solution
solution (ready-to-use), suitable for ELISA
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
BCIP®/NBT
SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet
MTT
Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
BM Blue POD Substrate, soluble
solution (ready-to-use), suitable for ELISA, pkg of 100 mL
Alkaline Phosphatase Yellow
alkaline phosphatase substrate, chromogenic liquid
Peroxidase from horseradish
Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Fast Green FCF
Dye content ≥85 %, powder
o-Phenylenediamine dihydrochloride
chromogenic, tablet
3,3′-Diaminobenzidine
SIGMAFAST™, chromogenic, tablet
4-Nitrophenyl phosphate disodium salt hexahydrate
chromogenic, ≥99.0% (enzymatic), powder or crystals
Related Resources
- Substrate Systems for Enzyme Detection
Convenient detection systems for Immunohistology, Western Blotting, and ELISA.
Peroxidase Enzymes
Our peroxidase enzymes are recognized around the world as the industry standard for diagnostic manufacturing and laboratory-scale research applications. Horseradish peroxidase (HRP) enzymes, peroxidase enzyme products, horseradish peroxidase substrates, and inhibitors are widely used as secondary detection reagents in ELISAs, immuno-histochemical techniques, and blotting analyses. In addition, you can conjugate our HRP to your own molecule including antibodies and avidins. Our peroxidase products include variations of HRP enzyme and chromogenic substrates of HRP such as 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA and SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablet. Other products include chemiluminescence substrates of HRP for ultra-sensitive detection such as Lumi-LightPLUS Western Blotting Substrate and BM Chemiluminescence ELISA Substrate (POD).
Alkaline Phosphatase (ALP) Enzymes
Our reliable ALPs are widely used in immunohistochemical assays, northern, Southern, and western blot applications, as well as ELISA-based diagnostics. Explore our ALP resource to find the right protocol for your assay needs. We offer a variety of ALP products and alkaline phosphatase substrates. Options include colorimetric substrates and chromogenic substrates such as phosphatase substrate tablets, SIGMAFAST™ BCIP®/NBT tablet, and Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA ready-to-use solution. Histochemical substrates include naphthol AS-TR phosphate disodium salt and 5-bromo-4-chloro-3-indolyl phosphate disodium salt. Additionally, we provide chemiluminescence substrates for ultra-sensitive detection such as CDP-Star®, ready-to-use disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′chloro)tricyclo [3.3.1.13,7 ] decan}-4-yl)-1-phenyl phosphate from Roche.
Luciferase Reagents
Luciferase is the original source for bioluminescent technology and firefly luciferase is a widely used bioluminescent reporter. These highly sensitive luciferase enzyme products are used extensively in molecular and cell biology, and for the efficient detection and quantitation of ATP as a reporter for genetic function. The light-producing reaction is initiated by D-luciferin activation. ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. We provide multiple formats of luciferase such as solutions and powders to fit your bioluminescent experiment needs. To protect the firefly population, we offer highly active green recombinant firefly luciferase. We also offer variations of the luciferase substrate, D-luciferin for luciferase assays.
Additional Detection Substrates
Our broad collection of additional detection substrates has numerous applications to suit your needs. This includes substrates for staining and labeling of actin microfilaments and the detection of cholesterol and lipoproteins in cells. Explore enzyme-based detection systems for ELISA, immunohistochemistry, and Western blotting including OPD, TMB, BCIP, pNPP, fast red, ABTS, DAB, chemiluminescence, and many more. We manufacture basic reagent powders as well as convenient solutions and tablets. We also offer enhancers and stop solutions specifically formulated for optimal performance with our detection substrates.
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