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Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Refolding of proteins using gel ﬁltration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Multisample Filtration of Small Volumes Using the Samplicity® System

This is an Application Note for multisample filtration of small volumes using the Samplicity® system

Gastrointestinal Organoid Biobank

Browse over 50 patient-derived human intestinal organoids from our PDO biobank. Various ages, sex, tissue origin and diseases available (normal, tumor organoids, ulcerative colitis).

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

HPLC determination of Besifloxacin in Ophthalmic Suspension using Chromolith^® SpeedROD RP-18e Column

A new HPLC method is proposed for the determination of Besifloxacin, a fluoroquinolone antibiotic in ophthalmic suspension using a Chromolith® SpeedROD RP-18e column. Read now!

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of

Cinchona Alkaloids

Asymmetric phase-transfer catalysis (PTC) has been recognized as a “green” alternative to many homogeneous synthetic organic transformations, and has found widespread application. Synthetically modified cinchona alkaloids are typical chiral organocatalysts used in asymmetric PTC.

Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Supelco SLB-IL60 Ionic Liquid GC Columns: Better High Temperature Stability

Benefits of higher temperature limits over PEG columns include decreased analysis times, elevated bake-out, and analysis of additional higher boiling compounds.

Tutorial in Microelectronics and Nanoelectronics

As the electronic industry continues to move towards smaller and smaller devices, researchers must continually strive for technologies to deposit electronic materials with precision at ever-smaller scales. This trend has lead to a variety of techniques used in the microelectronics

Cytiva™

Cytiva™ products for drug discovery research and therapeutics bioprocess manufacturing including Whatman™ lab filtration products, Amersham™ chromatography columns and resins, tissue culture reagents, and products for protein sample preparation, blotting, and analysis.

Functional RAFT Polymers for Biomedical Applications

Reversible addition–fragmentation chain transfer (RAFT) polymerization is rapidly moving to the forefront in construction of drug and gene delivery vehicles.

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.

Phosphoprotein Phosphatases (Serine/Threonine)

We offers many products related to phosphoprotein phosphatases (Serine/Threonine) for your research needs.

SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

Nanocomposite Coatings with Tunable Properties Prepared by Atomic Layer Deposition

Long and Accurate PCR Amplification of DNA with RedAccuTaq®

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

Quantitative Analysis of Nitrosamine Impurities by LC-MS Methods from the United States Pharmacopeia General Chapter <1469>

This application note describes the LC-MS-based quantitative analysis of known nitrosamine impurities following procedures 1 and 3 as given in USP general chapter .

The Utility of Headspace Grade Solvents for the Analysis of Organic Volatile Impurities

Fluorescein Isothiocyanate-Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

Silylethyne-Substituted Pentacenes

Primer Concentration Optimization Protocol

Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

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