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Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Separation Options with Superdex®
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
SYBR® Green JumpStart™ Taq ReadyMix™
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Sample Preparation for Chromatographic Purification
This page discusses various aspects of sample preparation for chromatographic purification.
Nanocomposite Coatings with Tunable Properties Prepared by Atomic Layer Deposition
Nanocomposite Coatings with Tunable Properties Prepared by Atomic Layer Deposition
Quantitative Analysis of Nitrosamine Impurities by LC-MS Methods from the United States Pharmacopeia General Chapter <1469>
This application note describes the LC-MS-based quantitative analysis of known nitrosamine impurities following procedures 1 and 3 as given in USP general chapter .
Fluorescein Isothiocyanate-Dextran
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
Perfluorosulfonic Acid Membranes for Fuel Cell and Electrolyser Applications
Advances in the electrochemical conversion of water to and from hydrogen and oxygen have principally been achieved through the development of new materials and by understanding the mechanisms of the degradation of proton exchange membrane fuel cells (PEMFC) during operation.
Graphene Inks for Printed Electronics
The emerging field of printed electronics requires a suite of functional materials for applications including flexible and large-area displays, radio frequency identification tags, portable energy harvesting and storage, biomedical and environmental sensor arrays,5,6 and logic circuits.
Bio-Inspired “Green” Synthesis of Nanomaterials and their Applications
Silica is a very popular inorganic nanomaterial used in a wide range of applications including fillers for rubber, catalyst supports, separation media, carriers in food and agriculture, and abrasive/anticaking agents in cosmetics. It is also widely believed to be an
Virus Cultivation
Cell lines for vaccine production and cultivation of SARS CoV-2 and other viruses. Primary, human diploid, and continuous culture cell lines for the propagation of viruses and virus-based vaccines.
Dextran and Related Polysaccharides
Learn about C6H10O6 or dextran formation, classes and naming. Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, which have a linear backbone of α-linked d-glucopyranosyl repeating units.
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
GABAB Receptors
We offer many products related to GABAB receptors for your research needs.
Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
Achieving High Efficiency in Organic Light-Emitting Devices
Electroluminescence of organic molecules has been a wellknown phenomenon for more than 50 years.
Aggregation-induced Emission Luminogens for Non-doped Organic Light-emitting Diodes
Organic Light-emitting Diodes (OLEDs) are solid-state devices that transform electrical energy into light. OLEDs are considered the next generation technology for high-resolution flexible displays and solid state lighting, attracting intense scientific and industrial interest.
Recent Advances on Nanoparticle-Based Imaging Contrast Agents for in Vivo Stem Cell Tracking
Professor Yu Cheng and co-workers look at the recent advances of nanoparticle-based imaging contrast agents for in vivo stem cell tracking. They provide an in-depth look at popular in vivo imaging techniques, magnetic resonance imaging, fluorescence imaging, ultrasound imaging, and
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Poly(2-Oxazoline)s: The Versatile Polymer Platform for Biomedicine
The introduction of polymers into the biomedical field has opened new avenues in tissue engineering, implant design, biosensing, and drug delivery.
Biomedical Implant Devices Fabricated from Low Young’s Modulus Titanium Alloys Demonstrating High Mechanical Biocompatibility
Biomedical implants are essentially foreign substances within the human body that must survive many years’ exposure to demanding mechanical and physiological conditions. Despite these challenges, metal implants have been widely used to substitute for or rebuild hard tissues such as
GPCR Markers for Cancer Research
Explore the role of some selected GPCR markers in cancer research development and treatment, including neurohormonal GPCRs, rhodopsin-like GPCRs, and more.
2D MXenes Ink: Synthesis, Properties, and Their Precursors
Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.
Delivery of Nucleic Acids Using Polymers
This review will focus on the sub-sector of drug delivery concerning the delivery of nucleic acids, with a particular focus on poly(ethyleneimine).
Immunohistochemistry: Current Applications in Skin Cancer
Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.
Lysing Enzymes
Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that
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