DNA and RNA purification
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Applications:DNA and RNA purification
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DNA Cleanup using illustra™ GFX Kits
DNA cleanup using illustra™ GFX kits from Cytiva.
Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.
RNA Stabilization at Room Temperature in RNAstable®
Quantitative RT-PCR analysis demonstrates successful amplification of RNA templates that were stored dry in RNAstable® for 29 months at room temperature and under accelerated aging conditions equivalent to 12 years of room temperature storage.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Biological Sample Collection, Transport, Archiving, and Purification for Nucleic Acid Analysis
A number of options are available for sample collection, transport, archiving, and DNA purification.
Plasmid DNA Purification using illustra™ plasmidPrep Mini Spin Kit
This page shows Plasmid DNA purification using illustra™ plasmidPrep Mini Spin Kit from Cytiva.
Genomic DNA Purification from Sample Applied to FTA Cards
This page shows genomic DNA purification from sample applied to FTA cards from Cytiva.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Whole Genome Amplification: Extraction Protocol - Blood Card
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides
Genomic DNA Purification from Sample on FTA Elute
This page shows genomic DNA purification from sample on FTA elute from Cytiva.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
GenElute™ HP Plasmid Maxiprep Procedure
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Plasmid DNA Preparation
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Instructions for EasiCollect™
This document shows intructions for EasiCollect™ device from Cytiva.
Deoxyribonuclease I
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
RNase, DNase-free Protocol
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
Plant/Fungi DNA Isolation Kit Protocol
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
Plasmid DNA Purification
Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. Various methods have been developed for plasmid DNA purification.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
GenElute™ RNA/DNA/Protein Plus Purification Kit
GenElute RNA/DNA/Protein Plus Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
Viral RNA Purification Protocol Using GenElute™ Mammalian Total RNA Purification Kits
Instructions for purifying viral RNA using GenElute™ Mammalian Total RNA Purification Kits.
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Reliable DNA extraction from Whatman™ FTA cards
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
GenElute™ PCR Clean-Up Procedure
Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.
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