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Enzyme activity assays

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Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)
Malic dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site that is critical for activity. This protocol uses a spectrophotometric assay to evaluate malic dehydrogenase activity.
Enzymatic Assay of Hyaluronidase (3.2.1.35)
This procedure may be used for Hyaluronidase products.
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Assay Procedure for Pyruvate Oxidase Bacterial
Protocol for Assay Procedure for Pyruvate Oxidase Bacterial
Assay Procedure for Glycerol Dehydrogenase
Assay Procedure for Glycerol Dehydrogenase
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
IFN-γ ELISpot Assays on MultiScreen® IP
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Enzymatic Assay of Invertase
Enzymatic Assay of Invertase
Assay Procedure for Lipase
Assay Procedure for Lipase
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Thrombin from Bovine Plasma
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Neuraminidase
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Enzymatic Assay of Cholesterol Oxidase
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product
Enzymatic Assay of Peroxidase (EC 1.11.1.7)
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Recycling Micro-Assay of β-NAD and β-NADH
Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH
Lentiviral Titer via p24 (viral coat protein) ELISA Assay
Calculate your lentiviral titer with the p24 ELISA assay. Detailed protcol below.
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
Enzymatic Method for Determining Alanine (Alanine Assay)
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)
Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Assay Procedure for Invertase
The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
Enzymatic Assay of Pectinase
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
MTT Assay Protocol for Cell Viability and Proliferation
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
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