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Delivery of Nucleic Acids Using Polymers
Delivery of Nucleic Acids Using Polymers
miRNA (microRNA) Introduction
Mature microRNAs (miRNAs) are a class of naturally occurring, small non-coding RNA molecules, about 21-25 nucleotides in length. MicroRNAs are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to downregulate gene expression...
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
esiRNA FAQs (Frequently Asked Questions)
Custom & Predesigned esiRNA FAQ
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
Genome-wide Collection of Lenti 3’UTR Reporters for Functional Validation of microRNA Targets
Sigma-Aldrich presents an article about microRNAs (miRNAs), which are a class of naturally occurring small non-coding RNA molecules that regulate a variety of developmental and physiological processes.
Discovery at Single-Cell Resolution
CRISPR lentiviral screening libraries are powerful research tools. Continuing a decade long commitment to library screening manufacturing, we have partnered with 10x Genomics to bring 10x Feature Barcode-compatible pooled screening products to you.
MISSION® Target ID Library for Human miRNA Target ID and Discovery
MISSION® Target ID Library for Human miRNA Target Identification and Discovery;
Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library
Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
Target Identification & Validation for Early Drug Discovery
Target identification, validation and characterization for early stages of drug discovery. Identifying drug target via genetic association, expression profiling.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library
The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.
MISSION™ shRNA Library: Next Generation RNA Interference
Work and refinement of the RNAi technology has exploded in recent years. We now know the basic mechanism of the endogenous RNAi pathway, that the pathway is present in most eukaryotes,3 and how cellular machinery can be harnessed to silence...
Methods for Enhancing Lentiviral Transduction Efficiency
An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.
MISSION® siRNA FAQs
We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.
Predesigned siRNA
We offer advanced design and quality synthesis for predesigned siRNA and siRNA libraries using the Rosetta siRNA Design Algorithm to reduce off-target effects and increase RNAi performance. The algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the siRNA seed...