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Showing 1-30 of 88 results
Evaluation of LCMSMS Deuterium Scrambling in Clinically Significant Small Molecules
LC-MS/MS is a powerful tool that brings numerous benefits to the clinical sample analysis arena. However, due to the complexity of the instrumentation there are some unique challenges that also accompany these benefits. Even following sample extraction and cleanup, matrix...
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
Characterization of Stable Isotope Caffeine as Reference Material for Isotope Dilution Mass Spectrometry
This technique is based on the direct proportionality of mass fraction ratio and signal intensity ratio of the natural isotope and the C-13 labeled caffeine.
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular...
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the...
Improved GC/MS Derivatization Techniques for Analysis of New Designer Drugs: Methylone, Ethylone, Butylone, Mephedrone, and Methedrone
As new “bath salt” drugs such as methylone, ethylone, mephedrone, butylone and other methcathinone analogs increase in popularity, toxicologists require native and labeled certified reference materials to accurately identify and quantify the new compounds in patient samples.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Extraction of Nitroimidazoles from Milk and Eggs using Molecularly Imprinted Polymers
In this report, SupelMIP SPE – Nitroimidazoles was evaluated for the extraction of nitroimidazoles and metabolites from milk and egg.
Extended Lifetime Ethanol Metabolites and Method Development for Ethyl Sulfate and Ethyl Glucuronide Separation
Review of clinically relevant ethanol metabolites and analytical method development for the analysis of ethyl glucuronide and ethyl sulfate in urine matrix.
Class-Selective Enantiomeric Separation of beta-Blockers and beta-Agonists using CHIROBIOTIC T Stationary Phase
Understanding the role of chirality in pharmacology became extremely important following the tragedy of thalidomide use in the 1960s. At that time the effect of chirality was poorly understood, and whilst it was discovered that the sedative effect resided in...
Hopane Reference Standards for use as Petrochemical Biomarkers in Oil Field Remediation and Spill Analysis
We provide high-quality, relevant hopane reference standards to be used as biomarkers for petrochemical oil field remediation and spill analysis.
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation
Vitamin D deficiency has become a topic of interest in recent publications. Vitamin D regulates the absorption of calcium within the digestive track and is important to promote proper bone growth and prevent osteoprosis in older adults.
Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals
Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.
Analysis of Pesticides in Baby Food by Solid Phase Microextraction (SPME) Using a New Overcoated Fiber
A direct immersion SPME method using a new overcoated SPME fiber was developed for the extraction of pesticides from pureed prune baby food.
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Flame Retardant Analytical Standards
Flame Retardant Standards: PBDE Standards. Polybrominated diphenyl ether standards accompany our chromatography products for this important analysis.
Online Solid Phase Extraction and LC/MS Analysis of Thyroid Hormones in Human Serum
An online SPE-LC/MS method has been developed for the rapid detection of thyroid hormones in human serum with minimal hands on effort and time-consuming steps.
A MALDI-based Chip Technology for Single-Cell Mass Spectrometry (SCMS)
Cell-to-cell variability can be due to a number of reasons; e.g., non-uniformity of the cellular environment, enetic differences resulting from mutations or stochastic events in gene expression. Here, We presenting the new MAMS for single-cell analysis.
Ultra-Pure MALDI Matrices
One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples...
HybridSPE®-Phospholipid Technology
Innovative solid phase extraction (SPE) technology from Supelco, utilizes phospholipid retention mechanism based on highly selective interaction between proprietary zirconia ions (bonded to the stationary phase) and the phosphate moiety consistent with phospholipids and proteins.
Molecularly Imprinted Polymer SPE Increases Sensitivity for the Extraction of Clenbuterol from Urine
In this report, we demonstrated the extraction of SupelMIP SPE – Clenbuterol method against a published hydrophilic polymer SPE method for the trace extraction of clenbuterol from urine for subsequent LC-MS-MS analysis.
Method Validation for Determination of Chloramphenicol in Urine Using Molecularly Imprinted Polymer (MIP) SPE
The method for determination of low concentrations of chloramphenicol in bovine and porcine urine was developed. Sample cleanup was performed by Molecular Imprinted Polymer (MIP) columns. Confirmatory analyses were conducted using GC/MS-NCI after derivatisation (silylation). The described method was fully...
Certified Reference Materials: Synthesis and Characterization of Cocaine N-oxide HCl
Cocaine is one of the most widespread illicit drugs of abuse in the US. Cocaine N-oxide is a metabolite of cocaine formed by cytochrome P-450 mediated oxidation of cocaine.1,2
Molecularly Imprinted Polymer SPE- Acid NSAIDs from Muscle
A new, successfully developed and validated LC-MS-MS method allowing the extraction of NSAIDs from poultry muscle tissue using Molecularly Imprinted Polymer (MIP) SPE.
Methods and matrices for mass spectrometry of glycans
Mass Spectrometry of Glycans, method comparison and products
Quantification of bitter acids in hops by thin layer chromatography
HPTLC separation followed by UV detection of the bitter acid content in various hop samples.
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
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