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Applications:Protein mass spectrometry
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Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
NMR Analysis of Glycans
Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.
Ion Chromatography for Sugar Testing in Food and Environmental Analysis
In this article we demonstrate how well ion chromatography is suited as an analytical technique for sugar analysis e.g. in apple juice, milk, and dust samples.
Quantification of Host Cell Proteins
Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
Development and Manufacturing of ADCs and Bio-Conjugates
As an industry leader in the conjugation of biological molecules with Highly-Active Pharmaceutical Ingredients (HAPIs), SAFC is among a handful of companies that can carry out the complexities of conjugation of biological molecules with HAPIs.
Chymotrypsin
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates
Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
Universal Proteomics Standards – Validating the Future of Proteomics
The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and...
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis...
AQUA™ Peptides for Absolute Quantitation
AQUA Protein - Ordering Page
Mass Spectrometric Quantitation in Lipidomic Studies
Dr. Robert Murphy, professor at University of Colorado - Denver, discusses Mass Spectrometric Quantitation in Lipidomic Studies.
Mass Spectrometric Approaches to Lipidomic Studies
Dr. Robert Murphy, professor at University of Colorado - Denver, discusses Mass Spectrometric Approaches to Lipidomic Studies.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.
A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation
In this study, we developed a rapid trypsin digest kit that, at elevated temperatures, yielded reliable, reproducible results in less than 2 hours on a wide variety of substrates for mass spectrometry.