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Removal of Detergents from Biological Product Matrices
The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation for Size Exclusion Chromatography
Performing a Separation with Superose
Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
Performing a Separation of Fibronectin with Gelatin Sepharose® 4B
This page shows how to separate fibronectin by affinity chromatography using Gelatin SepharosePerforming a Separation of Fibronectin with Gelatin Sepharose® 4B from Cytiva.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Purification using StrepTrap™ HP 1 mL and 5 mL
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Affinity Chromatography in a Purification Strategy (CIPP)
This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.
Characteristics of Glutathione Sepharose® Products
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Purification or Removal of Phosphorylated Biomolecules
This page shows how to Purify or remove phosphorylated biomolecules with a TiO2 Mag Sepharose from Cytiva.
Coupling Small Ligands Through Carboxyl Groups via a Spacer Arm
Ligands are coupled in a simple one-step procedure in the presence of a coupling reagent, carbodiimide. The carbodiimides may be regarded as anhydrides of urea.
Performing a Separation with Superdex
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Performing a Separation with Sephadex®
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum...
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Buffer Exchange and Desalting for Affinity Chromatography
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and...
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Performing a Separation with Sephacryl®
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Capto Core 700
Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
Chromatofocusing: Principles and Methods
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
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