• Home
  • Search Results
  • Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex.

Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex.

Molecular cell (2019-06-16)
Christophe Chapard, Robert Jones, Till van Oepen, Johanna C Scheinost, Kim Nasmyth

Cohesin entraps sister DNAs within tripartite rings created by pairwise interactions between Smc1, Smc3, and Scc1. Because Smc1/3 ATPase heads can also interact with each other, cohesin rings have the potential to form a variety of sub-compartments. Using in vivo cysteine cross-linking, we show that when Smc1 and Smc3 ATPases are engaged in the presence of ATP (E heads), cohesin rings generate a "SMC (S) compartment" between hinge and E heads and a "kleisin (K) compartment" between E heads and their associated kleisin subunit. Upon ATP hydrolysis, cohesin's heads associate in a different mode, in which their signature motifs and their coiled coils are closely juxtaposed (J heads), creating alternative S and K compartments. We show that K compartments of either E or J type can entrap single DNAs, that acetylation of Smc3 during S phase is associated with J heads, and that sister DNAs are entrapped in J-K compartments.

Product Number
Product Description

Triton X-100, laboratory grade
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Sodium citrate dihydrate, ≥99%, FG
Nocodazole, ≥99% (TLC), powder
Indole-3-acetic acid sodium salt, BioReagent, suitable for plant cell culture, ≥98%
Anti-Myc Tag Antibody, clone 4A6, clone 4A6, Upstate®, from mouse
Goat Anti-Rat light chain Antibody, HRP conjugate, Chemicon®, from goat