• Home
  • Search Results
  • Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells.

Mouse Rif1 is a key regulator of the replication-timing programme in mammalian cells.

The EMBO journal (2012-08-02)
Daniela Cornacchia, Vishnu Dileep, Jean-Pierre Quivy, Rossana Foti, Federico Tili, Rachel Santarella-Mellwig, Claude Antony, Geneviève Almouzni, David M Gilbert, Sara B C Buonomo

The eukaryotic genome is replicated according to a specific spatio-temporal programme. However, little is known about both its molecular control and biological significance. Here, we identify mouse Rif1 as a key player in the regulation of DNA replication timing. We show that Rif1 deficiency in primary cells results in an unprecedented global alteration of the temporal order of replication. This effect takes place already in the first S-phase after Rif1 deletion and is neither accompanied by alterations in the transcriptional landscape nor by major changes in the biochemical identity of constitutive heterochromatin. In addition, Rif1 deficiency leads to both defective G1/S transition and chromatin re-organization after DNA replication. Together, these data offer a novel insight into the global regulation and biological significance of the replication-timing programme in mammalian cells.

Product Number
Product Description

Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A, ascites fluid
Hygromycin B from Streptomyces hygroscopicus, powder, BioReagent, suitable for cell culture, suitable for insect cell culture
(Z)-4-Hydroxytamoxifen, ≥98% Z isomer
Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker, Upstate®, from rabbit
Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, ascites fluid
Nuclease micrococcal from Staphylococcus aureus, 100-300 units/mg protein