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A rapid and environmental friendly determination of the dithiocarbamate metabolites ethylenethiourea and propylenethiourea in fruit and vegetables by ultra high performance liquid chromatography tandem mass spectrometry.

Journal of chromatography. A (2011-06-17)
Aurore Bonnechère, Vincent Hanot, Joris Van Loco
ABSTRACT

Previous published methods for the analysis of ETU and PTU are time-consuming and furthermore use dichloromethane (DCM) for extraction or clean-up. This study details the development and validation of a rapid method that combines a simple extraction step with UHPLC-ESI(+)-MS/MS. This is the first application of UHPLC-MS/MS to analyse these compounds. Besides that, we replaced DCM with a more environmental-friendly solvent. The analytical performance was evaluated with the analysis of spiked celery samples at 50 μg kg(-1) (LOQ) and 300 μg kg(-1). The recoveries were between 65% and 90% for ETU and between 71% and 127% for PTU with RSDs in repeatability and reproducibility conditions below 10% for ETU. This method is rapid (a chromatographic run time of 2 min) and can easily be performed (no laborious clean-up). The presented method is environmental friendly with significant reduction in solvent consumption.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Imidazolidinethione, purum, ≥98.0% (HPLC)
Sigma-Aldrich
2-Imidazolidinethione, 98%
Supelco
2-Imidazolidinethione, PESTANAL®, analytical standard
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Zineb, PESTANAL®, analytical standard
Sigma-Aldrich
3,4,5,6-Tetrahydro-2-pyrimidinethiol, ≥99%