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Determination of active substances in binary mixture antiparasitic veterinary formulations by HPLC.

Acta poloniae pharmaceutica (2011-07-30)
Anna Kulik, Aleksandra Szczotkowska, Wanda Białecka, Marzena Podolska, Barbara Kwiatkowska-Puchniarz, Aleksander Mazurek
ABSTRACT

The purpose of the study was to develop a simple, versatile HPLC method for the identification and quantification of praziquantel and ivermectin (in Equimax) or praziquantel and abamectin (in Abamitel Plus). A satisfactory separation was obtained using the Supelcosil LC-ABZ+ column in gradient system with a mobile phase A: acetonitrile / water in 40:60 ratio and phase B: acetonitrile. The UV detection was set at 245 nm. The correlation coefficient values (> or = 0,998) for all active substances confirmed that the calibration curves (peak area vs. concentration) are linear. The results of the quantification and the statistical evaluation confirmed that the method is accurate and precise. It can also be applied to confirm the identity of benzyl alcohol, methyl p-hydroxybenzoate and propyl p-hydroxybenzoate in Abamitel Plus formulation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methyl 4-hydroxybenzoate, tested according to Ph. Eur.
Sigma-Aldrich
Propyl 4-hydroxybenzoate, tested according to Ph. Eur.
Sigma-Aldrich
Propyl 4-hydroxybenzoate, BioXtra
Sigma-Aldrich
Methyl 4-hydroxybenzoate, ≥99%, FCC
Sigma-Aldrich
Methyl 4-hydroxybenzoate, BioXtra, ≥99.0% (titration)
Sigma-Aldrich
Methyl 4-hydroxybenzoate, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Methyl 4-hydroxybenzoate, analytical standard
Sigma-Aldrich
Methyl 4-hydroxybenzoate, ReagentPlus®, ≥99.0%, crystalline
Sigma-Aldrich
Propyl 4-hydroxybenzoate, ≥99%
Supelco
Propylparaben, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Abamectin, PESTANAL®, analytical standard
Supelco
Methylparaben, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
SUPELCOSIL LC-ABZ Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Methyl Paraben, PESTANAL®, analytical standard
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
SUPELCOSIL LC-DABS HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Propyl parahydroxybenzoate, European Pharmacopoeia (EP) Reference Standard
Methyl parahydroxybenzoate, European Pharmacopoeia (EP) Reference Standard