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Brassica napus hairy roots and rhizobacteria for phenolic compounds removal.

Environmental science and pollution research international (2012-09-11)
Paola S González, Ornella M Ontañon, Ana L Armendariz, Melina A Talano, Cintia E Paisio, Elizabeth Agostini
ABSTRACT

Phenolic compounds are contaminants frequently found in water and soils. In the last years, some technologies such as phytoremediation have emerged to remediate contaminated sites. Plants alone are unable to completely degrade some pollutants; therefore, their association with rhizospheric bacteria has been proposed to increase phytoremediation potential, an approach called rhizoremediation. In this work, the ability of two rhizobacteria, Burkholderia kururiensis KP 23 and Agrobacterium rhizogenes LBA 9402, to tolerate and degrade phenolic compounds was evaluated. Both microorganisms were capable of tolerating high concentrations of phenol, 2,4-dichlorophenol (2,4-DCP), guaiacol, or pentachlorophenol (PCP), and degrading different concentrations of phenol and 2,4-DCP. Association of these bacterial strains with B. napus hairy roots, as model plant system, showed that the presence of both rhizospheric microorganisms, along with B. napus hairy roots, enhanced phenol degradation compared to B. napus hairy roots alone. These findings are interesting for future applications of these strains in phenol rhizoremediation processes, with whole plants, providing an efficient, economic, and sustainable remediation technology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Guaiacol, natural, ≥99%, FG
Sigma-Aldrich
Guaiacol, oxidation indicator
Supelco
Guaiacol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
2,4-Dichlorophenol, 99%
Sigma-Aldrich
Pentachlorophenol, 97%
Supelco
Pentachlorophenol, analytical standard
Supelco
Pentachlorophenol solution, certified reference material, 5000 μg/mL in methanol
Supelco
Pentachlorophenol solution, certified reference material, 500 μg/mL in methanol
Supelco
2,4-Dichlorophenol, PESTANAL®, analytical standard