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Anticancer activity of a hydrogel containing folic acid towards MCF-7 and MDA-MB-231 cells.

Anticancer research (2013-11-14)
Sonia Trombino, Teresa Ferrarelli, Michele Pellegrino, Emilia Ricchio, Loredana Mauro, Sebastiano Andò, Nevio Picci, Roberta Cassano
ABSTRACT

The aim of the present study was to prepare a hydrogel, based on ellagic acid and glycine, embedded with folic acid, as a subcutaneous implant for the treatment of breast cancer. The function of folic acid is to selectively and actively target tumor cells which are well-known to overexpress folic acid receptors on their surface. A pro-drug based on L-glycine and ellagic acid, was functionalized with a polymerizable group and loaded with folic acid to make it more natural, non-toxic, compatible and specific for the site of action. Cytotoxicity against MCF-7 cells was also evaluated. Release studies of folic acid were conducted on aliquots of hydrogel at different pH (6.2 and 7.4) and time-points (1, 6, 12 and 24 h) using a shaking water bath at 37°C (body temperature). Our results show that folic acid release by the hydrogel is characterized by a slow kinetic release, especially at pH 6.2. Moreover, it was evidenced that the exposure of human breast cancer cells to ellagic acid-based hydrogel containing folic acid significantly reduced cell viability.

MATERIALS
Product Number
Brand
Product Description

Supelco
Folic acid, Pharmaceutical Secondary Standard; Certified Reference Material
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Poly(ethylene glycol) dimethacrylate, average Mn 10,000, contains MEHQ as inhibitor
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Poly(ethylene glycol) dimethacrylate, average Mn 20,000, contains MEHQ as inhibitor
Supelco
Ellagic acid, analytical standard
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Ellagic acid, ≥95% (HPLC), powder, from tree bark
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Poly(ethylene glycol) dimethacrylate, average Mn 550, contains 80-120 ppm MEHQ as inhibitor, 270-330 ppm BHT as inhibitor
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Poly(ethylene glycol) dimethacrylate, average Mn 750, contains 900-1100 ppm MEHQ as inhibitor