Merck
  • Home
  • Search Results
  • A G-quadruplex DNAzyme-based colorimetric method for facile detection of Alicyclobacillus acidoterrestris.

A G-quadruplex DNAzyme-based colorimetric method for facile detection of Alicyclobacillus acidoterrestris.

The Analyst (2014-07-06)
Tao Liu, Xiao Zhang, Wenxin Zhu, Wei Liu, Daohong Zhang, Jianlong Wang
ABSTRACT

The rapid and sensitive detection of Alicyclobacillus acidoterrestris (AA) has become very important due to the frequent occurrence of fruit juice spoilage by AA. In the present study, using guaiacol, both as the metabolic product of AA related to its concentration and as a green colorimetric substrate of G-quadruplex DNAzyme, a novel G-quadruplex DNAzyme-based colorimetric method for a rapid detection of AA has been developed for the first time. Under optimal conditions, AA has been successfully detected in the concentration range of 10(2)-10(5) cfu mL(-1) with a detection limit of 85 cfu mL(-1). The recoveries ranging from 71.8% to 115.7% with relative standard deviation from 1.2% to 6.6% in spiked apple and orange juice samples were obtained. Results demonstrate that the sensitivity and precision of the developed method is comparable with most other analytical methods and is prominently rapid than them. We believe that the work provides a novel and effective approach and is beneficial for monitoring and reducing the risk of AA contaminations during the process of fruit juice production.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Guaiacol, natural, ≥99%, FG
Sigma-Aldrich
Hemin, from bovine, ≥90%
Supelco
Guaiacol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Hemin, BioXtra, from Porcine, ≥96.0% (HPLC)
Sigma-Aldrich
Guaiacol, oxidation indicator