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In vitro and in vivo anti-cancer effects of targeting and photothermal sensitive solid lipid nanoparticles.

Journal of drug targeting (2014-06-26)
Xiali Zhu, Shengnan Huang, Heqing Huang, Yingjie Zhang, Yingxia Xie, Lin Hou, Huijuan Zhang, Jinjin Shi, Zhenzhong Zhang
ABSTRACT

This report focuses on the in vitro and in vivo anti-cancer effects evaluation of targeting and photothermal sensitive solid lipid nanoparticles (SLN) for the co-loading of docetaxel (DTX), oxidized single-walled carbon nanotubes (OSWNT) and folic acid (FA). Compared with free DTX, FA-DTX-OSWNT-SLN could efficiently cross cell membranes and afford higher antitumor efficacy on the human hepatic carcinoma cell line (SMMC-7721) in vitro. Annexin V-FITC/PI double staining further confirmed that FA-DTX-OSWNT-SLN induced higher apoptotic rates on SMMC-7721 cells. Meanwhile, the combination of near-infrared (NIR) laser irradiation at 808 nm with FA-DTX-OSWNT-SLN significantly enhanced cell inhibition. For in vivo experiments, the relative tumor volume of the sarcomaia 180 (S180) tumor-bearing mice in FA-DTX-OSWNT-SLN group was significantly smaller than that of control groups. Furthermore, when combining with NIR laser irradiation, the suppression on tumor growth was much stronger. In a word, FA-DTX-OSWNT-SLN in combination with 808 nm NIR laser could exhibit both in vitro and in vivo anti-tumor effects and may be a promising approach for cancer therapy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Docetaxel, purum, ≥97.0% (HPLC)
Sigma-Aldrich
Sulforhodamine B, Dye content 75 %
USP
Docetaxel, United States Pharmacopeia (USP) Reference Standard
Anhydrous Docetaxel, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Sulforhodamine B sodium salt, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Sulforhodamine B sodium salt, Technical grade
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.6%, ReagentPlus®
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Supelco
Dimethyl sulfoxide, analytical standard
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Folic acid, meets USP testing specifications
Sigma-Aldrich
Folic acid, ≥97%
Sigma-Aldrich
Folic acid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥97%
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
USP
Folic acid, United States Pharmacopeia (USP) Reference Standard
Folic acid, European Pharmacopoeia (EP) Reference Standard
Supelco
Folic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%