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Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane.

Plant science : an international journal of experimental plant biology (2014-03-25)
Rafaela Ribeiro Reis, Bárbara Andrade Dias Brito da Cunha, Polyana Kelly Martins, Maria Thereza Bazzo Martins, Jean Carlos Alekcevetch, Antônio Chalfun, Alan Carvalho Andrade, Ana Paula Ribeiro, Feng Qin, Junya Mizoi, Kazuko Yamaguchi-Shinozaki, Kazuo Nakashima, Josirley de Fátima Corrêa Carvalho, Carlos Antônio Ferreira de Sousa, Alexandre Lima Nepomuceno, Adilson Kenji Kobayashi, Hugo Bruno Correa Molinari

Drought is one of the most challenging agricultural issues limiting sustainable sugarcane production and, in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory roles in abiotic stress responses in plants. The transcription factor DREB2A interacts with a cis-acting DRE sequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in Arabidopsis thaliana. In the present study, we evaluated the effects of stress-inducible over-expression of AtDREB2A CA on gene expression, leaf water potential (ΨL), relative water content (RWC), sucrose content and gas exchanges of sugarcane plants submitted to a four-days water deficit treatment in a rhizotron-grown root system. The plants were also phenotyped by scanning the roots and measuring morphological parameters of the shoot. The stress-inducible expression of AtDREB2A CA in transgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. The transgenic plants maintained higher RWC and ΨL over 4 days after withholding water and had higher photosynthetic rates until the 3rd day of water-deficit. Induced expression of AtDREB2A CA in sugarcane increased sucrose levels and improved bud sprouting of the transgenic plants. Our results indicate that induced expression of AtDREB2A CA in sugarcane enhanced its drought tolerance without biomass penalty.

Product Number
Product Description

Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
Sucrose, United States Pharmacopeia (USP) Reference Standard
Sucrose, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.5% (GC)
Sucrose, Grade I, suitable for plant cell culture
Sucrose, Grade II, suitable for plant cell culture
Sucrose, European Pharmacopoeia (EP) Reference Standard
Sucrose, ≥99.5%
Sucrose, ACS reagent
Sucrose, analytical standard, for enzymatic assay kit SCA20
Sucrose, BioXtra, ≥99.5% (GC)
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sucrose, analytical standard
Sucrose, ≥99.5% (GC)
Sucrose, for molecular biology, ≥99.5% (GC)
Sucrose, ACS reagent, suitable for microbiology, ≥99.0%
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF