Merck
  • Home
  • Search Results
  • Antioxidant and anticholinesterase activities of five wild mushroom species with total bioactive contents.

Antioxidant and anticholinesterase activities of five wild mushroom species with total bioactive contents.

Pharmaceutical biology (2015-02-28)
Gulsen Tel, Mehmet Ozturk, Mehmet E Duru, Aziz Turkoglu
ABSTRACT

Recently, mushrooms are interesting natural products to be investigated due to exhibiting various bioactivities. This study determines the antioxidant and anticholinesterase activities of various extracts of five wild mushroom species. In addition, the total bioactive contents, namely, ascorbic acid, β-carotene, and lycopene along with phenolic and flavonoid contents were also determined spectrophotometrically. Antioxidant activity was tested by using five complementary tests; namely, β-carotene-linoleic acid, DPPH(•) scavenging, ABTS(•+) scavenging, cupric-reducing antioxidant capacity (CUPRAC), and metal chelating assays. The in vitro anticholinesterase activity was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. The spectrophotometric methods were used to determine the total phenolic, flavonoid, ascorbic acid, β-carotene, and lycopene contents. The current study has shown that ethyl acetate extracts of Ganoderma lucidum (Curtis) P. Karst (IC50: 1.55 ± 0.05 µg/mL) and Funalia trogii (Berk.) Bondartsev & Singer (IC50: 4.31 ± 0.18 µg/mL) exhibited good lipid peroxidation inhibitory activity. The DPPH, ABTS, and CUPRAC assays supported this activity. The ethyl acetate and methanol extracts of Funalia trogii and Ganoderma lucidum indicated good anticholinesterase activity. Ganoderma lucidum had rich phenolic and flavonoid contents, indicating 98.67 ± 0.32 mg PEs/g extract and 160.38 ± 1.25 mg QEs/g extract, respectively. The results demonstrate that some of the mushroom species tested herein could be used in food and pharmaceutical industries as natural antioxidants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
Sigma-Aldrich
Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder
Sigma-Aldrich
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)
Sigma-Aldrich
Acetylthiocholine iodide, ≥98% (TLC), powder or crystals
Supelco
Ethyl acetate, analytical standard
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, for molecular biology, pH 8.0, ~0.5 M in H2O
Supelco
Anisole, analytical standard
Sigma-Aldrich
Acetylthiocholine iodide, ≥99.0% (AT)
Sigma-Aldrich
2,2-Diphenyl-1-picrylhydrazyl
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Sigma-Aldrich
Anisole, anhydrous, 99.7%
Sigma-Aldrich
Ethyl acetate, natural, ≥99%, FCC, FG
Sigma-Aldrich
Ethyl acetate, ≥99%, FCC, FG
Sigma-Aldrich
Hexane, anhydrous, 95%
Sigma-Aldrich
Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethyl acetate, anhydrous, 99.8%
Sigma-Aldrich
Iron(II) chloride, AnhydroBeads, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
Sigma-Aldrich
Ethylenediaminetetraacetic acid, ≥98.0% (KT)
Supelco
Hexane, analytical standard
Sigma-Aldrich
1,1-Diphenyl-2-picrylhydrazine, 97%
Sigma-Aldrich
Iron(II) chloride, AnhydroBeads, −10 mesh, 99.9% trace metals basis
Supelco
Ascorbic Acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Anisole, ≥99%, FCC, FG
Sigma-Aldrich
1,2-Dihydroxybenzene, ReagentPlus®, ≥99%
Sigma-Aldrich
Iron(II) chloride, AnhydroBeads, −10 mesh, 99.998% trace metals basis
Sigma-Aldrich
Ethyl acetate, ReagentPlus®, ≥99.8%
Sigma-Aldrich
Iron(II) chloride, 98%
Sigma-Aldrich
Ethylenediaminetetraacetic acid, 99.995% trace metals basis