Porous graphitic carbon (PGC) particles, designed by a unique and patented synthetic process, constitute the packing of Supel™ Carbon LC U/HPLC columns. The key advantages of using PGC columns over silica particle-packed columns include:
Supel™ Carbon LC Specifications
The Supel™ Carbon LC columns are available in internal diameters of 2.1, 3.0, and 4.6 mm with guard cartridges and holders available for each inner diameter.
Figure 1. Scanning electron microscope (SEM) image of the Supel™ Carbon LC particles. Notice the narrow particle size distribution
The patented synthetic process used for the synthesis of Supel™ Carbon LC columns generates a narrow particle size distribution (PSD). The resulting narrow PSD leads to a higher efficiency due to the reduction in brand broadening caused by eddy diffusion effects.
One application demonstrating the Supel™ Carbon LC’s excellent resolving power is the separation of Vitamins D2 and D3 metabolites. These metabolites are polar and structurally similar, making their chromatographic separation difficult on the conventional silica based reversed-phase columns. The Supel™ Carbon LC column resolves the metabolites with high efficiency.
Figure 2.Chromatographic separation of Vitamin D2 and D3 metabolites on Supel™ Carbon LC. Conditions: Column: Supel™ Carbon LC, 10 cm x 2.1 mm I.D., 2.7 µM; Mobile Phase: [A] 2-Propanol; [B] Tetrahydrofuran; Gradient: 0% B to 70% B in 15 min; hold at 70% B for 5 min; Flow Rate: 0.3 mL/min; Column Temp.: 25 °C; Detector: UV, 275 nm; Injection: 2.0 µL; Sample: Vitamin D2 and D3 metabolites mix, varied concentration, ethanol
Supel™ Carbon LC columns are compatible with all HPLC, UHPLC, UPLC, and LC-MS instruments.
Application note: LC-MS/MS Analysis of 20 Underivatized Amino Acids on Supel™ Carbon LC Column
Application note: UHPLC-MS/MS Analysis of Polar Pesticides on the Supel™ Carbon LC Column
Application note: UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Column