The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples. To be suitable for further analysis, isolated organelles must be relatively pure, and structurally and functionally intact. It is also important to verify the organelle integrity with robust assays.
We offer several organelle isolation and enrichment kits, companion organelle functionality assays, and antibodies that are suitable for evaluation of samples from plant, animal, and cell culture sources.