937835

Protein delivery system

GFP, PODS^®

• Synonyme(s) : PODS^®
• NACRES: NA.21

Propriétés

• biological source: (Spodoptera frugiperda (Sf9) cell culture)
• Quality Segment: 100
• form: (monodisperse crystals)
• dilution: 3D bioprinting | cell culture
• impurities: <0.06 EU/mL Endotoxin
• color: colorless
• storage temp.: 2-8°C

Description

General description

POlyhedrin delivery systems (PODS) are a class of delivery systems specifically designed for the efficient delivery of proteins to target cells or tissues. They offer a platform to overcome the challenges associated with protein delivery, such as stability, cell membrane permeability, and targeted delivery. Protein delivery system , GFP, PODS contains a unique versatile biomarker, polyhedrin protein co-crystalized with green fluorescent protein (GFP) encased into PODS crystals. GFP offers a simple way to visualize and localize PODS crystals embedded in biomaterials, such as hydrogels and scaffolds, using fluorescence microscopy. PODS GFP crystals can be excited at 488 nm and optimally detected at 510 nm, compatible with commonly available filter sets.

Application

PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein.PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.

PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.

Features and Benefits

• The product is a monomer with molecular weight of 32.1 kDa; endotoxin levels: <0.06 EU/ml as measured by gel clot LAL assay.
• This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
• PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS^® co-crystals and can be useful for aliquoting. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
• PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.

Legal Information

PODS is a registered trademark of Cell Guidance Systems Ltd

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable