14-743-M

Sigma-Aldrich

GCK Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

Synonyme(s):
germinal center kinase
eCl@ss:
32160405
NACRES:
NA.32

Niveau de qualité

100

Source biologique

human

specific activity

2337 U/mg

Poids mol.

Mw 80.6 kDa

manufacturer/tradename

Upstate®

Application(s)

activity assay: suitable (kinase)

N° d'accession NCBI

N° d'accession UniProt

Description générale

N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
Product Source: Recombinant protein expressed in Sf21 cells

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Actions biochimiques/physiologiques

Target Sub-Family: STE
Protein Target: GCK

Qualité

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.

Définition de l'unité

Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

Forme physique

Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.

Stockage et stabilité

Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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