AB3849-I

Sigma-Aldrich

Anti-phospho SMAD2 Antibody (Ser465/467)

from rabbit, purified by affinity chromatography

Synonyme(s):
Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated, hMAD-2, Ser465/467 phosphorylated, hSMAD2, Ser465/467 phosphorylated, JV18-1, Ser465/467 phosphorylated, MAD homolog 2, Ser465/467 phosphorylated, Mad-related protein 2, Ser465/467 pho
eCl@ss:
32160702
NACRES:
NA.41

Source biologique

rabbit

Niveau de qualité

100

antibody product type

primary antibodies

Clone

polyclonal

Purifié(e)(s) par

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

Application(s)

western blot: suitable

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... SMAD2(4087)

Description générale

Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.

Spécificité

This antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).

Immunogène

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
Epitope: Phospho-Ser465/467.

Application

Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.

Qualité

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.

Description de la cible

~53 kDa 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.

Liaison

Replaces: AB3849

Forme physique

Affinity Purfied
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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