ABN1676M

Sigma-Aldrich

Anti-Calcipressin-1

serum, from rabbit

Synonyme(s):
RCAN-1, Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1
eCl@ss:
32160702
NACRES:
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

serum

antibody product type

primary antibodies

Clone

polyclonal

species reactivity

human, rat

Conditionnement

antibody small pack of 25 μL

Application(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG

N° d'accession NCBI

N° d'accession UniProt

Catégories apparentées

Description générale

Calcipressin-1 (Uniprot: Q6IN33; also known as Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN-1) is encoded by the Rcan1 (also known as Dscr1) gene (Gene ID: 266766) in rat. Calcipressin-1 is a cytoplasmic protein that directly binds to the catalytic domain of calcineurin and acts as its negative regulator. When phosphorylated at the conserved FLISPP motif its capacity to inhibit calcineurin is increased. This phosphorylation also controls the half-life of calcipressin-1 by accelerating its degradation. It is a calcium-responsive enzyme, which is reported to dephosphorylate the nuclear factor of activated T cells (NFAT), which promotes its nuclear translocation and links calcium signaling to transcriptional regulation. Calcipressin-1 displays a quick turnover with a half-life of less than 3 hours. However, a Ser to Ala mutation at Ser163 prolongs its half-life to more than 9 hours. (Ref.: Genesca, L et al. (2003). Biochem. J. 374(2); 567-575).

Spécificité

This rabbit polyclonal antibody detects calcipressin-1 (RCAN-1) in human and rat cells. It targets an epitope with in 11 amino acids from the C-terminal half.

Immunogène

A linear peptide corresponding to 11 amino acids from the C-terminal half of rat Calcipressin-1.
Epitope: unknown

Application

Western Blotting Analysis: A representative lot detected Calcipressin-1 in cultured rat sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).

Immunocytochemistry Analysis: A representative lot detected Calcipressin-1 in sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).
Research Category
Neuroscience
Anti-Calcipressin-1 Antibody, Cat. No. ABN1676, is a highly specific rabbit polyclonal antibody that targets Calcipressin-1 and has been tested in Immunocytochemistry and Western Blotting.

Qualité

Evaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Calcipressin-1 in SH-SY5Y cells.

Description de la cible

22.95 kDa calculated.

Forme physique

Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
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