Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03

clone 3CSP03, Chemicon®, from mouse


Niveau de qualité


Source biologique


antibody product type

primary antibodies


3CSP03, monoclonal

species reactivity





immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable



N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... CASP3(836)


Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.


Recombinant full-length human caspase-3 protein
Epitope: large subunit & proform


Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
Western blot: 1 μg/ml for 2 hours at RT.

Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

Optimal working dilutions must be determined by end user.
Research Sub Category

Enzymes & Biochemistry
Research Category
Apoptosis & Cancer



Replaces: 04-440; 04-1088

Forme physique

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Format: Purified

Stockage et stabilité

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne


Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

M Vakkala et al.
British journal of cancer, 81(4), 592-599 (1999-11-26)
The aim of this investigation was to study the expression of caspases 3, 6 and 8 and their association to apoptosis in preneoplastic and neoplastic lesions of the breast. The material consisted of nine benign breast epithelial hyperplasias, 15 atypical...
Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma.
Virkajarvi, N, et al.
Histopathology, 33, 432-439 (1998)
Z Mallat et al.
Circulation, 96(2), 424-428 (1997-07-15)
Apoptosis that has been reported in human atherosclerosis may contribute to the remodeling of atherosclerotic plaques. The identification of specific markers for apoptosis in these plaques would permit the development of specific therapeutic strategies to limit their progression. Cysteine protease...
M Volm et al.
Anticancer research, 19(3A), 1669-1671 (1999-09-02)
In order to explore whether apoptosis is associated with angiogenesis in lung cancer, immunohistochemistry was employed to determine the pro-apoptotic factors Fas ligand (FasL) and caspase-3 (Cas-3) in 70 squamous cell lung carcinomas. Furthermore, the vascular endothelial growth factor (VEGF)...
Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
Krajewska, M, et al.
Cancer Research, 57, 1605-1613 (1997)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique

Réseaux sociaux

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Recherche. Développement. Production

Nous sommes un des tout premiers fournisseurs du secteur mondial des Sciences de la vie proposant des solutions et des services pour la recherche, le développement et la production en biotechnologies, et le développement et la production de produits thérapeutiques pharmaceutiques.

© 2021 Merck KGaA, Darmstadt, Allemagne et/ou ses sociétés affiliées. Tous droits réservés.

La reproduction d'une quelconque partie du contenu de ce site est strictement interdite sans autorisation.