MAB4603

Sigma-Aldrich

Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03

clone 3CSP03, Chemicon®, from mouse

Synonyme(s):
CPP32
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

3CSP03, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

Application(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2a

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... CASP3(836)

Spécificité

Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.

Immunogène

Recombinant full-length human caspase-3 protein
Epitope: large subunit & proform

Application

Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
Western blot: 1 μg/ml for 2 hours at RT.

Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

Optimal working dilutions must be determined by end user.
Research Sub Category
Caspases

Enzymes & Biochemistry
Research Category
Apoptosis & Cancer

Metabolism

Liaison

Replaces: 04-440; 04-1088

Forme physique

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Format: Purified

Stockage et stabilité

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 2

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

M Vakkala et al.
British journal of cancer, 81(4), 592-599 (1999-11-26)
The aim of this investigation was to study the expression of caspases 3, 6 and 8 and their association to apoptosis in preneoplastic and neoplastic lesions of the breast. The material consisted of nine benign breast epithelial hyperplasias, 15 atypical...
Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma.
Virkajarvi, N, et al.
Histopathology, 33, 432-439 (1998)
Z Mallat et al.
Circulation, 96(2), 424-428 (1997-07-15)
Apoptosis that has been reported in human atherosclerosis may contribute to the remodeling of atherosclerotic plaques. The identification of specific markers for apoptosis in these plaques would permit the development of specific therapeutic strategies to limit their progression. Cysteine protease...
M Volm et al.
Anticancer research, 19(3A), 1669-1671 (1999-09-02)
In order to explore whether apoptosis is associated with angiogenesis in lung cancer, immunohistochemistry was employed to determine the pro-apoptotic factors Fas ligand (FasL) and caspase-3 (Cas-3) in 70 squamous cell lung carcinomas. Furthermore, the vascular endothelial growth factor (VEGF)...
Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.
Krajewska, M, et al.
Cancer Research, 57, 1605-1613 (1997)

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