JMJD2C, also known as Lysine-specific demethylase 4C, and encoded by the gene KDM4C/ GASC1/ JHDM3C/ JMJD2C/ KIAA0780, is a histone demethylase that specifically demethylates ′Lys-9′ and ′Lys-36′ residues of histone H3, thereby playing a central role in histone code. JMJD2C does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, nor H4 ′Lys-20′. JMJD2C can also demethylate trimethylated H3 ′Lys-9′ and H3 ′Lys-36′ residues, but JMJD2C has no activity on mono- and dimethylated residues. JMJD2C is overexpressed in several esophageal squamouse cancer cells and serves as a cancer marker for such cancers. This gene is also amplified in squamous cell carcinoma 1 protein (GASC-1). By demethylating H3K9me, JMJD2C/KDM4C acts as a transcription promoter on many target genes including genes the affect cell growth in normal or cancer cells. JMJD2C also regulates sphere formation by mediating the cross talk between Wnt and Notch pathways in colonic cancer cells and thus is a target for anti-cancer therapy for this disease.
GST-tagged recombinant protein corresponding to human JMJD2C.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JMJD2C in HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies.
Anti-JMJD2C Antibody, clone 6A3.1 is an antibody against JMJD2C for use in western blotting & ICC.
Epigenetics & Nuclear Function
Research Sub Category
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected JMJD2C in 10 µg of HeLa neclear extract.
Description de la cible
~120 kDa observed
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
ALEXA FLUOR is a registered trademark of Life Technologies
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.