MABF546

Sigma-Aldrich

Anti-CD25 Antibody (human), PE, clone BC96

clone BC96, from mouse

Synonyme(s):
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD antigen CD25
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

BC96, monoclonal

species reactivity

human

Conditionnement

pkg of 5 μL (0.06 μg/test)

Application(s)

flow cytometry: suitable

Isotype

IgG1κ

Conjugué

PE

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... IL2RA(3559)

Description générale

The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.
The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD25 (human), PE & clone BC96 validated for use in Flow Cytometry for the detection of CD25.

Qualité

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD25 in one million human peripheral blood lymphocytes.

Forme physique

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Stockage et stabilité

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

nwg

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

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