MABN328

Sigma-Aldrich

Anti-peptidyl-citrulline, clone F95 Antibody

clone F95, from mouse

Synonyme(s):
Peptidyl-citrulline, Protein-bound citrulline
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

F95, monoclonal

species reactivity

human, mouse, rat, chicken

species reactivity (predicted by homology)

all (based on 100% sequence homology)

Application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgMκ

Expédié(e)(s) dans

wet ice

Description générale

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

Immunogène

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

Application

This Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Qualité

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.

Description de la cible

Various kDa calculated

Forme physique

Purified by Ion-Exchange Chromatography
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

R Bright et al.
Journal of periodontal research, 53(1), 139-143 (2017-10-19)
Postranslational modification of proteins can lead to the production of autoantibodies and loss of immune tolerance. This process has been hypothesised to be a critical factor in the pathogenesis of rheumatoid arthritis. The objective of this study was to demonstrate...
Sanna Turunen et al.
Arthritis research & therapy, 18(1), 239-239 (2016-10-22)
Seropositive rheumatoid arthritis (RA) is characterized by autoantibodies binding to citrullinated and homocitrullinated proteins. We wanted to study the expression patterns of these disease-associated protein forms and if the rheumatoid nodule and synovial tissue itself contain biologically active levels of...
Expression of citrullinated proteins in murine experimental autoimmune encephalomyelitis.
Nicholas, Anthony P, et al.
The Journal of Comparative Neurology, 486, 254-266 (2005)
Dual immunofluorescence study of citrullinated proteins in Parkinson diseased substantia nigra.
Nicholas, Anthony P
Neuroscience Letters, 495, 26-29 (2011)
Leen De Rycke et al.
Arthritis and rheumatism, 52(8), 2323-2330 (2005-07-30)
To address the ongoing debate concerning the specificity of synovial citrullinated proteins for rheumatoid arthritis (RA) and to analyze their pathophysiologic relevance to the induction or perpetuation of the RA-specific anti-citrullinated protein antibodies (ACPAs). Synovium of 19 RA patients and...

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