MABN497

Sigma-Aldrich

Anti-PSD93 Antibody, clone N18/30

clone N18/30, from mouse

Synonyme(s):
Disks large homolog 2, Channel-associated protein of synapse-110, Chapsyn-110, Postsynaptic density protein PSD-93
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

N18/30, monoclonal

species reactivity

rat, human, mouse

Application(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1κ

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Description générale

As a member of the membrane-associated guanylate kinase (MAGUK) family, PSD93 (also known as Chapsyn-110) binds directly to the N-methyl-D-aspartate (NMDA) receptor and the Shaker K+ channel subunits. It shares 70-80% homology with PSD-95 (SAP90), and SAP97. Like PSD-95, PSD93 plays a key role in postsynaptic targeting. Aside from binding to glutamate receptors, the MAGUK family of proteins also interacts with cell surface structures, like cell adhesion molecules, ion channels, cytoskeletal elements, and signal transduction molecules. Improper PSD93 signaling has been implicated in certain disease states, such as schizophrenia.

Spécificité

This antibody does not demonstrate cross reactivity with other MAGUK family members, such as PSD-95, SAP97, and SAP102 expressed in transfected cells.

Immunogène

Recombinant protein corresponding to rat PSD93.

Application

Western Blot Analysis: 1 µg/mL from a representative lot detected PSD93 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A representative lot detected PSD93 in rat brain tissue (Prof. J. Trimmer, University of California, Davis).

Western Blot Analyis: A representative lot detected PSD93 in WT, but not in PSD93 knockout mice.

Western Blot Analysis: A representative lot detected PSD93 in CA1 tissue lysates from C57BL/6 mice (Jeffrey, R. A., et al. (2009). J Neurosci. 29(50):15613-15620.).
Research Category
Neuroscience
Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC.
Research Sub Category
Developmental Signaling

Qualité

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PSD93 in 10 µg of mouse brain tissue lysate.

Description de la cible

~110 kDa observed. The calculated molecular weight of PSD93 is 95 kDa; however, this protein is highly phosphorylated and is observed at ~110 kDa Multiple isoforms between 68-98 kDa are known to exist.

Forme physique

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse brain tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Rachel A Jeffrey et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(50), 15613-15620 (2009-12-18)
Synaptic plasticity, the capacity of neurons to change the strength of their connections with experience, provides a mechanism for learning and memory in the brain. Long-term plasticity requires new transcription, indicating that synaptically generated signals must be transported to the...
Austin A Coley et al.
Scientific reports, 9(1), 9486-9486 (2019-07-03)
Postsynaptic density protein-95 (PSD-95) is a major regulator in the maturation of excitatory synapses by interacting and trafficking N-methyl-D-aspartic acid receptors (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isox-azoleproprionic acid receptors (AMPAR) to the postsynaptic membrane. PSD-95 disruption has recently been associated with neuropsychiatric disorders...

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