MABS489

Sigma-Aldrich

Anti-LRP-2/Megalin Antibody, clone 10D5.1

clone 10D5.1, from mouse

Synonyme(s):
Low-density lipoprotein receptor-related protein 2, Calcium sensor protein, Glycoprotein 330, gp330, LRP-2, Megalin
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

10D5.1, monoclonal

species reactivity

human

Application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotype

IgG1κ

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... LRP2(4036)

Description générale

Low-density lipoprotein receptor-related protein 2 (UniProt P98164; also known as Calcium sensor protein, Glycoprotein 330, gp330, Heymann nephritis antigen homolog, LRP-2, Megalin) is encoded by the LRP2 (also known as DBS) gene (Gene ID 4036) in human. Megalin/LRP-2 is a 600 kDa type I membrane receptor initially produced with a signal peptide sequence (a.a. 1-25). The mature receptor has a large extracellular region (a.a. 26-4423), a transmembrane segment (a.a. 4424-4446), and cytoplasmic tail (a.a. 4447-4655). Megalin belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the very-low-density lipoprotein (VLDL) receptor, the LDLR-related protein (LRP), and the apolipoprotein E receptor 2 (ApoER2). The overall extracellular structural features are similar among all LDLR family members. Megalin contains four clusters of cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding. These are separated by 17 EGF-like repeats and eight cysteine-poor spacer regions that contain YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments. Megalin is a multiligand, endocytic receptor expressed primarily in the apical membranes of polarized epithelial cells. In addtion to functioning alone, megalin is also reported to interact with another structurally distinct endocytic receptor cubilin in several tissues where they are co-expressed. The internalization of several cubilin ligands is reported to be strongly facilitated in the presence of megalin.

Immunogène

Epitope: LDL-receptor class A repeats 19-21.
GST-tagged recombinant protein corresponding to the LDL-receptor class A repeats 19-21 of human LRP-2/Megalin.

Application

Research Sub Category
Lipid Signaling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected LRP-2/Megalin in 10 µg of human kidney tissue lysate.
Research Category
Signaling

Qualité

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LRP-2/Megalin in human kidney tissue.

Description de la cible

~250 to 600 kDa observed. A broad/smeared banding pattern is consistent with the detection of the target protein with varying degrees of glycosylation (520 kDa/non-glycosylated to 660 kDa/fully glycosylated), as well as smaller size degradation products.

Forme physique

Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine
M R Morgan et al.
Oncogene, 30(12), 1422-1435 (2010-12-07)
Expression of the integrin αvβ6 is upregulated in a variety of carcinomas where it appears to be involved in malignant progression, although the biology of this integrin is not fully explored. We have generated oral carcinoma cells that express αvβ6...
Annika N Alexopoulou et al.
Breast cancer research : BCR, 12(4), R51-R51 (2010-07-21)
Breast cancer currently accounts for more than one-quarter of all female cancers and, despite the great progress in treatment observed in the past few years, the need for identification of new gene targets that can be used for diagnosis, prognosis...
Liudmila S Chesnokova et al.
Journal of virology, 88(21), 12193-12201 (2014-08-22)
Epstein-Barr virus (EBV) fusion with an epithelial cell requires virus glycoproteins gHgL and gB and is triggered by an interaction between gHgL and integrin αvβ5, αvβ6, or αvβ8. Fusion with a B cell requires gHgL, gp42, and gB and is...

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