MABT376

Sigma-Aldrich

Anti-Ero1 Antibody, clone 2G4/12

clone 2G4/12, from mouse

Synonyme(s):
ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha
eCl@ss:
32160702
NACRES:
NA.41

Niveau de qualité

100

Source biologique

mouse

antibody product type

primary antibodies

Clone

2G4/12, monoclonal

species reactivity

rat, mouse, human

Application(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2aκ

N° d'accession NCBI

N° d'accession UniProt

Expédié(e)(s) dans

wet ice

Gene Information

human ... ERO1L(30001)

Description générale

ERO1-like protein alpha (ERO1) is also called endoplasmic oxidoreductin-1-like protein and Oxidoreductin-1-L-alpha. ERO1 is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1 is stimulated by hypoxia, indicating that it is regulated by the HIF-pathway. ERO1 may be responsible for much of the reactive oxygen species (ROS) in the cell, and therefore is a source of oxidative stress. EROS1 is required for the folding of immunoglobulin proteins, responsible for the release of the unfolded cholera toxin, and plays an important role in ER stress-induced, CHOP-dependent apoptosis. ERO1 is widely expressed at low levels, with high levels in upper digestive tract.

Immunogène

GST-tagged recombinant protein corresponding to Human Ero1.

Application

Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 1 µg/mL from a representative lot detected Ero1 from 10 µg of the following cell lysates--A431, HEK293, HeLa, HepG2, human placenta tissue, HUVEC, jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ero1 in Ero1 transfected HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Immunofluorescence Analysis: A representative lot detected Ero1 in HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).
Anti-Ero1 Antibody, clone 2G4/12 is a highly specific mouse monoclonal antibody, that targets ERO1-like protein alpha & has been tested in western blotting, IP & Immunofluorescence.
Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ero1 in 10 µg of HEK293 cell lysate.

Description de la cible

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HEK293 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Allemagne

WGK 1

Flash Point(F)

Not applicable

Point d'éclair C

Not applicable

Certificat d'analyse
Certificat d'origine
null
Jianchao Zhang et al.
The EMBO journal, 37(14) (2018-06-03)
Family with sequence similarity 20C (Fam20C), the physiological Golgi casein kinase, phosphorylates numerous secreted proteins that are involved in a wide variety of biological processes. However, the role of Fam20C in regulating proteins in the endoplasmic reticulum (ER) lumen is...
Jingqi Fang et al.
Aging cell, 17(4), e12765-e12765 (2018-04-17)
Metformin, an FDA-approved antidiabetic drug, has been shown to elongate lifespan in animal models. Nevertheless, the effects of metformin on human cells remain unclear. Here, we show that low-dose metformin treatment extends the lifespan of human diploid fibroblasts and mesenchymal...
Xun Wu et al.
Redox biology, 20, 46-59 (2018-10-08)
Endothelial dysfunction induced by hyperhomocysteinemia (HHcy) plays a critical role in vascular pathology. However, little is known about the role of endoplasmic reticulum (ER) redox homeostasis in HHcy-induced endothelial dysfunction. Here, we show that Hcy induces ER oxidoreductin-1α (Ero1α) expression...

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